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Promega luciferase reporter gene vectors pmirglo
Luciferase Reporter Gene Vectors Pmirglo, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/luciferase+reporter+gene+vector/pmirglo+vector/10__2147_slash_jir__s507828-61-5-10
Average 90 stars, based on 1 article reviews
luciferase reporter gene vectors pmirglo - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Binding Assay:

Article Title: POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer
Article Snippet: .. The sequences of the binding sites (site 1 and site 2) were designed and synthesized for inserting into the luciferase reporter gene vector (pGL3-Basic, Promega, Madison, WI, USA) and cell transfection. .. The fluorescence intensity was measured using a dual luciferase reporter gene assay kit (Promega).

Article Title: MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1
Article Snippet: The possible target relationships between FHL1 and miR-183-5p/miR-96-5p were predicted via TargetScan. .. A sequence corresponding to the FHL1-3ʹ-UTR with the predicted miR-183-5p or miR-96-5p binding site was synthesized by RiboBio Co., Ltd. (Guangzhou, China) and cloned into a luciferase reporter gene vector (E1330; Promega, Madison, USA). .. The sequence of the putative miR-183-5p or miR-96-5p binding site in the FHL1-3ʹ-UTR was mutated, and FHL1-3ʹ-UTR-mut (sequence: 5ʹ-UGUAUUGUCCCGACUGCAAUAAA-3ʹ) was constructed using the QuickChange Site-Directed Mutagenesis kit (Stratagene, Cedar Creek, USA).

Synthesized:

Article Title: POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer
Article Snippet: .. The sequences of the binding sites (site 1 and site 2) were designed and synthesized for inserting into the luciferase reporter gene vector (pGL3-Basic, Promega, Madison, WI, USA) and cell transfection. .. The fluorescence intensity was measured using a dual luciferase reporter gene assay kit (Promega).

Article Title: MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1
Article Snippet: The possible target relationships between FHL1 and miR-183-5p/miR-96-5p were predicted via TargetScan. .. A sequence corresponding to the FHL1-3ʹ-UTR with the predicted miR-183-5p or miR-96-5p binding site was synthesized by RiboBio Co., Ltd. (Guangzhou, China) and cloned into a luciferase reporter gene vector (E1330; Promega, Madison, USA). .. The sequence of the putative miR-183-5p or miR-96-5p binding site in the FHL1-3ʹ-UTR was mutated, and FHL1-3ʹ-UTR-mut (sequence: 5ʹ-UGUAUUGUCCCGACUGCAAUAAA-3ʹ) was constructed using the QuickChange Site-Directed Mutagenesis kit (Stratagene, Cedar Creek, USA).

Luciferase:

Article Title: POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer
Article Snippet: .. The sequences of the binding sites (site 1 and site 2) were designed and synthesized for inserting into the luciferase reporter gene vector (pGL3-Basic, Promega, Madison, WI, USA) and cell transfection. .. The fluorescence intensity was measured using a dual luciferase reporter gene assay kit (Promega).

Article Title: MicroRNA-125a-5p regulates liver cancer cell growth, migration and invasion and EMT by targeting HAX1
Article Snippet: .. The HAX1-3′-UTR mut was purchased from Guangzhou RiboBio Co., Ltd. and cloned into a luciferase reporter gene vector (cat. no. E1330; Promega Corporation). .. For the dual-luciferase reporter assay, the reporter vector plasmid and miR-125a mimics or miR-223 mimics were co-transfected into 293T cells (cat. no. CRL-11268; American Type Culture Collection) using LipofectamineTM 2000 (cat. no. 11668; Invitrogen; Thermo Fisher Scientific, Inc.).

Article Title: Abstracts for the 62nd Annual Meeting of the Society for Investigative Dermatology (abstracts 600-799)
Article Snippet: The sequences were analysed using the Transfac database (release 5.0) and prospective regulatory elements identi®ed. .. Portions of the regulatory region (1 kb, 2 kb and 3 kb) of Hb3 and Hb6 were subcloned into a luciferase reporter gene vector (Promega pGL3) and transfected into Cos cells. ..

Article Title: miRNA-192-5p impacts the sensitivity of breast cancer cells to doxorubicin via targeting peptidylprolyl isomerase A.
Article Snippet: .. This fragment was linked to the luciferase reporter gene vector (Promega). .. Mutagenesis of the binding sites was achieved using the Site-Directed Mutagenesis Kit (Toyobo, Japan).

Article Title: MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1
Article Snippet: The possible target relationships between FHL1 and miR-183-5p/miR-96-5p were predicted via TargetScan. .. A sequence corresponding to the FHL1-3ʹ-UTR with the predicted miR-183-5p or miR-96-5p binding site was synthesized by RiboBio Co., Ltd. (Guangzhou, China) and cloned into a luciferase reporter gene vector (E1330; Promega, Madison, USA). .. The sequence of the putative miR-183-5p or miR-96-5p binding site in the FHL1-3ʹ-UTR was mutated, and FHL1-3ʹ-UTR-mut (sequence: 5ʹ-UGUAUUGUCCCGACUGCAAUAAA-3ʹ) was constructed using the QuickChange Site-Directed Mutagenesis kit (Stratagene, Cedar Creek, USA).

Article Title: Circ_0134944 inhibits osteogenesis through miR-127-5p/PDX1/SPHK1 pathway
Article Snippet: Segments of circ_0134944 and the 3′UTR of PDX1 containing miR-127-5p binding sites were synthesized by Genepharma (Shanghai, China). .. These fragments were linked to the luciferase reporter gene vector (Promega, Madison, WI, USA). .. Mutagenesis of the binding sites in circ_0134944 and PDX1 were achieved using the Site-Directed Mutagenesis Kit (Toyobo, Japan).

Plasmid Preparation:

Article Title: POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer
Article Snippet: .. The sequences of the binding sites (site 1 and site 2) were designed and synthesized for inserting into the luciferase reporter gene vector (pGL3-Basic, Promega, Madison, WI, USA) and cell transfection. .. The fluorescence intensity was measured using a dual luciferase reporter gene assay kit (Promega).

Article Title: Abstracts for the 62nd Annual Meeting of the Society for Investigative Dermatology (abstracts 600-799)
Article Snippet: The sequences were analysed using the Transfac database (release 5.0) and prospective regulatory elements identi®ed. .. Portions of the regulatory region (1 kb, 2 kb and 3 kb) of Hb3 and Hb6 were subcloned into a luciferase reporter gene vector (Promega pGL3) and transfected into Cos cells. ..

Article Title: miRNA-192-5p impacts the sensitivity of breast cancer cells to doxorubicin via targeting peptidylprolyl isomerase A.
Article Snippet: .. This fragment was linked to the luciferase reporter gene vector (Promega). .. Mutagenesis of the binding sites was achieved using the Site-Directed Mutagenesis Kit (Toyobo, Japan).

Article Title: MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1
Article Snippet: The possible target relationships between FHL1 and miR-183-5p/miR-96-5p were predicted via TargetScan. .. A sequence corresponding to the FHL1-3ʹ-UTR with the predicted miR-183-5p or miR-96-5p binding site was synthesized by RiboBio Co., Ltd. (Guangzhou, China) and cloned into a luciferase reporter gene vector (E1330; Promega, Madison, USA). .. The sequence of the putative miR-183-5p or miR-96-5p binding site in the FHL1-3ʹ-UTR was mutated, and FHL1-3ʹ-UTR-mut (sequence: 5ʹ-UGUAUUGUCCCGACUGCAAUAAA-3ʹ) was constructed using the QuickChange Site-Directed Mutagenesis kit (Stratagene, Cedar Creek, USA).

Article Title: Circ_0134944 inhibits osteogenesis through miR-127-5p/PDX1/SPHK1 pathway
Article Snippet: Segments of circ_0134944 and the 3′UTR of PDX1 containing miR-127-5p binding sites were synthesized by Genepharma (Shanghai, China). .. These fragments were linked to the luciferase reporter gene vector (Promega, Madison, WI, USA). .. Mutagenesis of the binding sites in circ_0134944 and PDX1 were achieved using the Site-Directed Mutagenesis Kit (Toyobo, Japan).

Transfection:

Article Title: POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer
Article Snippet: .. The sequences of the binding sites (site 1 and site 2) were designed and synthesized for inserting into the luciferase reporter gene vector (pGL3-Basic, Promega, Madison, WI, USA) and cell transfection. .. The fluorescence intensity was measured using a dual luciferase reporter gene assay kit (Promega).

Article Title: Abstracts for the 62nd Annual Meeting of the Society for Investigative Dermatology (abstracts 600-799)
Article Snippet: The sequences were analysed using the Transfac database (release 5.0) and prospective regulatory elements identi®ed. .. Portions of the regulatory region (1 kb, 2 kb and 3 kb) of Hb3 and Hb6 were subcloned into a luciferase reporter gene vector (Promega pGL3) and transfected into Cos cells. ..

Clone Assay:

Article Title: MicroRNA-125a-5p regulates liver cancer cell growth, migration and invasion and EMT by targeting HAX1
Article Snippet: .. The HAX1-3′-UTR mut was purchased from Guangzhou RiboBio Co., Ltd. and cloned into a luciferase reporter gene vector (cat. no. E1330; Promega Corporation). .. For the dual-luciferase reporter assay, the reporter vector plasmid and miR-125a mimics or miR-223 mimics were co-transfected into 293T cells (cat. no. CRL-11268; American Type Culture Collection) using LipofectamineTM 2000 (cat. no. 11668; Invitrogen; Thermo Fisher Scientific, Inc.).

Article Title: MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1
Article Snippet: The possible target relationships between FHL1 and miR-183-5p/miR-96-5p were predicted via TargetScan. .. A sequence corresponding to the FHL1-3ʹ-UTR with the predicted miR-183-5p or miR-96-5p binding site was synthesized by RiboBio Co., Ltd. (Guangzhou, China) and cloned into a luciferase reporter gene vector (E1330; Promega, Madison, USA). .. The sequence of the putative miR-183-5p or miR-96-5p binding site in the FHL1-3ʹ-UTR was mutated, and FHL1-3ʹ-UTR-mut (sequence: 5ʹ-UGUAUUGUCCCGACUGCAAUAAA-3ʹ) was constructed using the QuickChange Site-Directed Mutagenesis kit (Stratagene, Cedar Creek, USA).

Sequencing:

Article Title: MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1
Article Snippet: The possible target relationships between FHL1 and miR-183-5p/miR-96-5p were predicted via TargetScan. .. A sequence corresponding to the FHL1-3ʹ-UTR with the predicted miR-183-5p or miR-96-5p binding site was synthesized by RiboBio Co., Ltd. (Guangzhou, China) and cloned into a luciferase reporter gene vector (E1330; Promega, Madison, USA). .. The sequence of the putative miR-183-5p or miR-96-5p binding site in the FHL1-3ʹ-UTR was mutated, and FHL1-3ʹ-UTR-mut (sequence: 5ʹ-UGUAUUGUCCCGACUGCAAUAAA-3ʹ) was constructed using the QuickChange Site-Directed Mutagenesis kit (Stratagene, Cedar Creek, USA).



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